Fig. 1.
D1αPan and D1βPan, but not D2αPan receptors were distributed in the lateral pyloric (LP) somatodendritic compartment. Wholemount stomatogastric ganglion (STG) preparations, each containing a single Texas red filled LP, were stained with anti-D1βPan (A–E), anti-D1αPan (F–I), or anti-D2αPan (J–L). n ≥ 5 wholemount preparations for each receptor type. Yellow staining indicates dopamine receptor (DAR) expression in the LP. Green staining represents DAR expression in unidentified cells. Merged confocal projections were made from serial 1 μm confocal optical slices. A: merged, 3 μm confocal projection from a wholemount preparation showing D1βPan receptor expression in LP perinuclear vesicles (yellow puncta) and unidentified cells (green puncta). Green tubular structures surrounding somata suggests D1βPan receptor expression in glial and /or other support cells. B: a 3 μm merged confocal projection from the center of the LP soma showing D1βPan receptor expression in the perinuclear vesicles. C: a 4 μm merged confocal projection from deep within the synaptic neuropil showing D1βPan receptors in cytoplasmic transport vesicles in higher order neurites (→). ▴, putative synaptic varicosities containing D1βPan receptors. D: high magnification 1 μm optical slice showing cytoplasmic transport vesicles in higher order neurite. E: high magnification 3–4 μm projection showing a cluster of LP synaptic terminals, some of which contain D1βPan receptors.→, the terminal lacking the red cytoplasmic ring structure. F–H: 4 μm merged confocal projections showing D1αPan receptor expression in the LP soma, primary neurite, higher order neurites and synaptic terminals (→). I: high magnification 1 μm optical slice showing the presence of D1αPan receptors on LP synaptic terminals. →, the terminals lacking a red cytoplasmic ring. J: a 37 μm merged confocal projection showing the absence of detectable D2αPan in the LP soma and primary neurites. Green staining represents D2αPan receptors in unidentified neurons. K: a 4 μm confocal projection from deep within the synaptic neuropil showing the absence of D2αPan in LP higher order neurites and terminals. L: high magnification 1 μm optical slice showing a cluster of LP synaptic terminals lacking D2αPanreceptors.