TABLE 1.

Sequences of primers used for detection of the bla_TLA-1 gene and its circular form with ISCR20 and for mapping the genetic environment

Primer name	Primer sequence	Positiona
TLA-1F	TGTGTGCTTTTTGCTTCTGC	1
TLA-1R	GCTTCCGGTTTTATGAGCAA	2
TLA-1-5′-TR1	TACTGCTTTTAAGCGAATCCG	3
TLA-1-5′-TR2	TTAGCCGCAAAAGCAGAAGC	4
TLA-1-5′-TR3	AAGCAAAAAGCACACAAAATGC	5
IS91-5′-TR1	ACAACCGACATTGACCGTGG	6
IS91-5′-TR4	CTAAAACATGCCAGTGCGGG	7
IS91-5′-TR5	AATGCTCTAGGCGGTCAAGG	8
IS91-5′-TR6	GACATGGCTTTTTGCTGGCG	9
IS91-5′-TR7	GATATTGCTTCGATGCAGCAC	10
TLA1-3′-TR1	CATTGCTGTTTATGTGTCGG	11
TLA1-3′-TR4	CATCGCGTCATTAATCCTGC	12
TLA1-3′-TR5	GATCGAACAGATTTCCGCCT	13
TLA1-3′-TR6	ACGAACAACAGAAAAGCGCC	14
IS91-3′extR	CTTTTGTAACAACCTTGCCC	15
TLA1-3′extF	GTATTATTACTCTGCCGAACGG	16
IS91-5′extR	TCTGAAGTACGAATGGCCAC	17
IS91-3′extF	ACCAAGATTATTGACTGGGC	18
ICR20F	CAAAGCTGATCGTGAAGCCC	19
Maturase F	AACATCATGCTCGACCCAC	20
Methyl R	CATCGAATTGAGCGAGTCG	21

^aPositions of the primers indicated in Fig. 1. All the primers were designed in this study.