Population analysis of PAO1 and PAΔnZ CAZ susceptibility. Overnight cultures of PAO1 or PAΔnZ were plated in MH agar containing 0, 0.5, 1, 2, 4, 8, and 16 μg/ml of CAZ; and the numbers of CFU were enumerated after 24 h of incubation. The results shown here are the mean values of three experiments ± standard deviations.