TABLE 1.

Strains and plasmids used or constructed

Strain or plasmid	Genotype/relevant characteristicsa	Reference or source
P. aeruginosa
PAO1	Reference strain completely sequenced	Laboratory collection
PAΔD	PAO1 ΔampD::lox	18
PAΔdB	PAO1 ΔdacB::lox	31
PAdacB	1A1 spontaneous dacB null mutant (W273X) of PAO1	31
PAdacBΔD	PAdacB ΔampD::lox	31
PAΔDDh2Dh3	PAO1 ΔampD::lox ΔampDh2::lox ΔampDh3::lox	18
PAΔR	PAO1 ΔampR::lox	31
PAΔC	PAO1 ΔampC::lox	30
PAΔnZ	PAO1 ΔnagZ::lox	This work
PAΔDnZ	PAO1 ΔampD::lox ΔnagZ::lox	This work
PAΔdBnZ	PAO1 ΔdacB::lox ΔnagZ::lox	This work
PAdacBΔDnZ	PAdacB ΔampD::lox ΔnagZ::lox	This work
PAΔDDh2Dh3Nz	PAO1 ΔampD::lox ΔampDh2::lox ΔampDh3::lox ΔnagZ::lox	This work
E. coli
XL1-Blue	F′::Tn10 proA+B+ lacIq Δ(lacZ)M15/recA1 endA1 gyrA96 (Nalr) thi hsdR17 (rk− mk−) mcrB1	Laboratory collection
S17-1	RecA pro (RP4-2Tet:: Mu Kan::Tn7)	Laboratory collection
Plasmids
pEX100Tlink	AprsacB, pUC19-based gene replacement vector with an MCS	37
PUCGmlox	Apr Gmr, pUC18-based vector containing the lox-flanked aacC1 gene	37
pCM157	Tcr, cre expression vector	37
PEXnZ	pEX100Tlink containing 5′ and 3′ flanking sequences of nagZ	This work
PEXnZGm	pEX100Tlink containing 5′ and 3′ flanking sequences of nagZ::Gmlox	This work

^aMCS, multiple cloning site; Ap^r, ampicillin resistant; Gm^r, gentamicin resistant; Tc^r, tetracycline resistant.