Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Primers used for construction of nagZ-knockout mutants
| Primer | Sequence (5′-3′)a | Locationb | PCR product size (bp) |
|---|---|---|---|
| nZF1-BHI | TCGGATCCTCCGGGCGGAACTCCATG | −153 to −135 | 505 |
| nZR1-Hd3 | TCAAGCTTAGCACCGGCGCGAAACTCA | 335 to 353 | |
| nZF2-Hd3 | TCAAGCTTAGCGACCTGGTTCCGTTCG | 568 to 586 | 432 |
| nZR2-ERI | TCGAATTCACTCATCGACAGCTCCCTCA | 980 to 999 |
The restriction sites for endonucleases are underlined.
Location of primers with respect to the nagZ start codon.
