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. 2001 Feb 6;98(4):1877–1882. doi: 10.1073/pnas.041592598

Figure 2.

Figure 2

(A) Photographic image of an immunoblot of infected cell proteins bound to GST or chimeric GST-BMAL1 fusion protein, electrophoretically separated in a denaturing gel and reacted with a mouse mAb to ICP0 (H1083). Lysates of infected HEp-2 were reacted with GST or GST-BMAL1 chimeric protein immobilized on glutathione-agarose beads. The beads were pelleted, rinsed extensively, subjected to electrophoresis on a denaturing gel, transferred to a nitrocellulose sheet, and reacted with the ICP0 antibody. Lines 1 and 2, whole-cell extracts (WCE) from HSV-1(F)-infected HEp-2 cells reacted with GST or GST-BMAL1, respectively; lanes 3 and 4, whole-cell extracts from HSV-1(F)-infected or mock-infected HEp-2 cells, respectively. Molecular weights (×1,000) are shown on the left. (B) Photographic image of an immunoblot of proteins extracted from transfected cells and bound to GCT, GST-α020–241, or GST-α0543–768, electrophoretically separated in a denaturing gel, and reacted with a mouse mAb to the Flag epitope (M2). Lysates of COS-7 cells transfected with pME-BMAL1(F) were reacted with GST or GST-ICP0 chimeric protein immobilized on glutathione-Sepharose beads. The beads were pelleted, rinsed extensively, subjected to electrophoresis on a denaturing gel, transferred to a nitrocellulose sheet, and reacted with the anti-Flag epitope antibody. Lanes 1, 2, and 3, whole-cell extracts from COS-7 cells transfected with pME-BMAL1(F) bound to GST, GST-α020–241, and GST-α0543–768, respectively; lane 4, whole-cell extracts from COS-7 cells transfected with pME-BMAL1(F). Molecular weights (103) are shown on the left.