TABLE 1.
Plasmids used and constructed in this study
| Plasmid | Relevant properties | Source or reference |
|---|---|---|
| pRSET/BFP | Source of bfp gene | Invitrogen |
| pECFP-C1 | Source of cfp gene | BD Biosciences |
| pEYFP-C1 | Source of yfp gene | BD Biosciences |
| p415_Gal1_RedStar | Source of redStar gene | 20 |
| pQE30 | Enables N-terminal fusion with 12-amino-acid leader containing a His6 tag; Ampr | Qiagen |
| pHGFP | gfp-mut2 cloned into BamHI-SacI sites of pQE30 | 30 |
| pHBFP | bfp cloned into BamHI-SacI sites of pQE30 | This study |
| pHCFP | cfp cloned into BamHI-SacI sites of pQE30 | This study |
| pHYFP | yfp cloned into BamHI-SacI sites of pQE30 | This study |
| pHRS | redStar cloned into BamHI-SacI sites of pQE30 | This study |
| pHGFP_CBD118-D | ply118 fragment encoding Asp90-Ile281 cloned into SacI-SalI sites of pHGFP | 30 |
| pHGFP_CBD500-A | ply500 fragment encoding His133-Lys289 cloned into SacI-SalI sites of pHGFP | 30 |
| pHGFP_CBDPSA | plyPSA fragment encoding Thr172-Lys314 cloned into SacI-SalI sites of pHGFP | 21 |
| pHGFP_CBD006-A | ply006 fragment encoding Glu135-Ile235 cloned into SacI-PstI sites of pHGFP | This study |
| pHGFP_CBD006-B | ply006 fragment encoding Lys115-Ile235 cloned into SacI-PstI sites of pHGFP | This study |
| pHGFP_CBD006-C | ply006 fragment encoding Leu90-Ile235 cloned into SacI-PstI sites of pHGFP | This study |
| pHGFP_CBDP35-A | plyP35 fragment encoding Phe167-Lys291 cloned into SacI-SalI sites of pHGFP | This study |
| pHGFP_CBDP35-B | plyP35 fragment encoding Glu150-Lys291 cloned into SacI-SalI sites of pHGFP | This study |
| pHGFP_CBDP35-C | plyP35 fragment encoding Pro130-Lys291 cloned into SacI-SalI sites of pHGFP | This study |
| pHGFP_CBD511-A | ply511 fragment encoding Gly177-Lys341 cloned into SacI-SalI sites of pHGFP | This study |
| pHGFP_CBD511-B | ply511 fragment encoding Ile161-Lys341 cloned into SacI-SalI sites of pHGFP | This study |
| pHGFP_CBD511-C | ply511 fragment encoding Lys143-Lys341 cloned into SacI-SalI sites of pHGFP | This study |
| pHGFP_CBDP40-A | plyP40 fragment encoding Pro291-Lys344 cloned into SacI-SalI sites of pHGFP | This study |
| pHGFP_CBDP40-B | plyP40 fragment encoding Gly201-Lys344 cloned into SacI-SalI sites of pHGFP | This study |
| pHGFP_CBDP40-C | plyP40 fragment encoding Asp187-Lys344 cloned into SacI-SalI sites of pHGFP | This study |
| pHGFP_CBDP40-D | plyP40 fragment encoding Gly158-Lys344 cloned into SacI-SalI sites of pHGFP | This study |
| pHGFP_CBD025-A | ply025 fragment encoding Lys144-Lys276 cloned into SacI-SalI sites of pHGFP | This study |
| pHGFP_CBD025-B | ply025 fragment encoding Thr132-Lys276 cloned into SacI-SalI sites of pHGFP | This study |
| pHGFP_CBD025-C | ply025 fragment encoding Met119-Lys276 cloned into SacI-SalI sites of pHGFP | This study |
| pHGFP_CBD025-D | ply025 fragment encoding Trp106-Lys276 cloned into SacI-SalI sites of pHGFP | This study |
| pHBFP_CBD118 | ply118 fragment encoding Asp90-Ile281 cloned into SacI-SalI sites of pHBFP | This study |
| pHBFP_CBD006 | ply006 fragment encoding Leu90-Ile235 cloned into SacI-PstI sites of pHBFP | This study |
| pHBFP_CBD500 | ply500 fragment encoding His133-Lys289 cloned into SacI-SalI sites of pHBFP | This study |
| pHBFP_CBDPSA | plyPSA fragment encoding Thr172-Lys314 cloned into SacI-SalI sites of pHBFP | This study |
| pHBFP_CBDP35 | plyP35 fragment encoding Pro130-Lys291 cloned into SacI-SalI sites of pHBFP | This study |
| pHBFP_CBD511 | ply511 fragment encoding Ile161-Lys341 cloned into SacI-SalI sites of pHBFP | This study |
| pHBFP_CBDP40 | plyP40 fragment encoding Gly158-Lys344 cloned into SacI-SalI sites of pHBFP | This study |
| pHBFP_CBD025 | ply025 fragment encoding Thr132-Lys276 cloned into SacI-SalI sites of pHBFP | This study |
| pHCFP_CBD118 | ply118 fragment encoding Asp90-Ile281 cloned into SacI-SalI sites of pHCFP | This study |
| pHCFP_CBD006 | ply006 fragment encoding Leu90-Ile235 cloned into SacI-PstI sites of pHCFP | This study |
| pHCFP_CBD500 | ply500 fragment encoding His133-Lys289 cloned into SacI-SalI sites of pHCFP | This study |
| pHCFP_CBDPSA | plyPSA fragment encoding Thr172-Lys314 cloned into SacI-SalI sites of pHCFP | This study |
| pHCFP_CBDP35 | plyP35 fragment encoding Pro130-Lys291 cloned into SacI-SalI sites of pHCFP | This study |
| pHCFP_CBD511 | ply511 fragment encoding Ile161-Lys341 cloned into SacI-SalI sites of pHCFP | This study |
| pHCFP_CBDP40 | plyP40 fragment encoding Gly158-Lys344 cloned into SacI-SalI sites of pHCFP | This study |
| pHCFP_CBD025 | ply025 fragment encoding Thr132-Lys276 cloned into SacI-SalI sites of pHCFP | This study |
| pHYFP_CBD118 | ply118 fragment encoding Asp90-Ile281 cloned into SacI-SalI sites of pHYFP | This study |
| pHYFP_CBD006 | ply006 fragment encoding Leu90-Ile235 cloned into SacI-PstI sites of pHYFP | This study |
| pHYFP_CBD500 | ply500 fragment encoding His133-Lys289 cloned into SacI-SalI sites of pHYFP | This study |
| pHYFP_CBDPSA | plyPSA fragment encoding Thr172-Lys314 cloned into SacI-SalI sites of pHYFP | This study |
| pHYFP_CBDP35 | plyP35 fragment encoding Pro130-Lys291 cloned into SacI-SalI sites of pHYFP | This study |
| pHYFP_CBD511 | ply511 fragment encoding Ile161-Lys341 cloned into SacI-SalI sites of pHYFP | This study |
| pHYFP_CBDP40 | plyP40 fragment encoding Gly158-Lys344 cloned into SacI-SalI sites of pHYFP | This study |
| pHYFP_CBD025 | ply025 fragment encoding Thr132-Lys276 cloned into SacI-SalI sites of pHYFP | This study |
| pHRS_CBD118 | ply118 fragment encoding Asp90-Ile281 cloned into SacI-SalI sites of pHRS | This study |
| pHRS_CBD006 | ply006 fragment encoding Leu90-Ile235 cloned into SacI-PstI sites of pHRS | This study |
| pHRS_CBD500 | ply500 fragment encoding His133-Lys289 cloned into SacI-SalI sites of pHRS | This study |
| pHRS_CBDPSA | plyPSA fragment encoding Thr172-Lys314 cloned into SacI-SalI sites of pHRS | This study |
| pHRS_CBDP35 | plyP35 fragment encoding Pro130-Lys291 cloned into SacI-SalI sites of pHRS | This study |
| pHRS_CBD511 | ply511 fragment encoding Ile161-Lys341 cloned into SacI-SalI sites of pHRS | This study |
| pHRS_CBDP40 | plyP40 fragment encoding Gly158-Lys344 cloned into SacI-SalI sites of pHRS | This study |
| pHRS_CBD025 | ply025 fragment encoding Thr132-Lys276 cloned into SacI-SalI sites of pHRS | This study |