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. 2010 Jul 9;76(17):5827–5836. doi: 10.1128/AEM.00106-10

FIG. 3.

FIG. 3.

SDS-PAGE analysis of recombinant BgpA. Lanes: M, molecular mass standard; 1, crude extract of C41(DE3) carrying pGEX-bgpA; 2, GST-BgpA enzyme fraction after glutathione-Sepharose chromatography; 3, enzyme fraction after DEAE anion-exchange chromatography and removal of the GST epitope by thrombin; 4, recombinant BgpA after Mono Q anion-exchange chromatography.