Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Purification scheme
| Purification step | Vol (ml) | Total protein (mg) | Sp act (U/mg) | Total activity (U)a | Yield (%) | Purification (fold) |
|---|---|---|---|---|---|---|
| Crude extract | 5 | 191.1 | 3.6 | 696 | 100 | 1.0 |
| Glutathione-Sepharose | 12 | 5.5 | 63.5 | 351 | 50 | 17.4 |
| DEAE-cellulose | 27 | 2.7 | 67.3 | 182 | 26 | 18.5 |
| Mono Q | 5 | 1.2 | 75.9 | 87 | 13 | 20.8 |
One unit of BgpA was defined as the amount of enzyme liberating 1 μmol/min of p-nitrophenol.
