TABLE 3.
Effects of metal ions and chemical agents on the activity of purified recombinant BgpA
| Metal ion or reagent | Relative activity ± SD (%) ata: |
|
|---|---|---|
| 1 mM | 10 mM | |
| NaCl | 102.3 ± 5.15 | 97.7 ± 4.51 |
| KCl | 102.7 ± 4.65 | 96.2 ± 4.64 |
| MgCl2 | 94.8 ± 5.10 | 95.2 ± 5.14 |
| MnCl2 | 101.1 ± 5.45 | 96.9 ± 4.79 |
| CoCl2 | 100.9 ± 8.43 | 97.0 ± 5.04 |
| ZnCl2 | 92.8 ± 4.72 | 72.8 ± 4.27 |
| CaCl2 | 91.8 ± 4.59 | 101.5 ± 5.23 |
| CuCl2 | 84.7 ± 4.48 | 71.7 ± 5.07 |
| HgCl2 | 0 | 0 |
| SDS | 94.8 ± 6.98 | 2.7 ± 3.22 |
| EDTA | 98.6 ± 6.49 | 96.6 ± 4.52 |
| β-Mercaptoethanol | 98.4 ± 4.89 | 102.5 ± 4.57 |
| DTT | 96.7 ± 5.59 | 96.6 ± 5.14 |
| Control | 100.0 ± 5.17 | 100.0 ± 5.11 |
a
The specific activity at 100% was 44.3 U/mg protein.