TABLE 4.

Relative activity of purified recombinant BgpA toward various chromogenic substrates as measured by ONP or PNP release at 37°C

Substrate	Relative activity ± SD (%)a
PNP-α-d-glucopyranoside	1.12 ± 0.21
PNP-α-d-mannopyranoside	0
PNP-α-d-xylopyranoside	0
PNP-α-l-arabinofuranoside	0
PNP-α-l-arabinopyranoside	0
PNP-α-l-rhamnopyranoside	0
PNP-β-d-fucopyranoside	6.98 ± 0.06
PNP-β-d-galactopyranoside	0
PNP-β-d-glucopyranoside	89.52 ± 4.43
PNP-β-d-glucosaminide	0
PNP-β-d-mannopyranoside	0
PNP-β-d-xylopyranoside	0
PNP-β-l-arabinopyranoside	0
ONP-α-d-galactopyranoside	0
ONP-β-d-fucopyranoside	15.88 ± 0.54
ONP-β-d-galactopyranoside	2.33 ± 0.08
ONP-β-d-glucopyranoside	100.00 ± 3.54

^aActivity toward ONP-β-d-glucopyranoside was set as 100% and corresponded to a specific activity of 44.3 U/mg.