TABLE 1.
Acids (solutes) used for experiments and key experimental data obtained
| Exptl group(s) | Acid | Chemical formula | Acid supplier | pKa | Tested pH range | No. of bins | No. of data points (no. excludeda) | Tested conc range (mM) | No. of bins | No. of data points (no. excludeda) | Conjugated salt | Salt supplier |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | Sulfuric acid | H2SO4 | Riedel-de Haën, Seelze, Germany | <1 | 4.60-7.46 | 56 | 598 (2; 0) | |||||
| 2, 3 | Acetic acid (HAc) | C2H4O2 | Merck KGaA, Darmstadt, Germany | 4.75 | 5.11-7.0 | 36 | 399 (0; 1) | 0-10 | 16 | 596 (3; 1) | Potassium acetate (KAc) | Merck KGaA, Darmstadt, Germany |
| 2, 3 | Lactic acid (HLa) | C3H6O3 | Sigma-Aldrich Chemie GmbH, Steinheim, Germany | 3.85 | 4.86-7.11 | 49 | 496 (7; 0) | 0-5 | 16 | 400 (0; 0) | Potassium lactate (KLa) | PURAC Biochem, Gorinchem, Netherlands |
| 2, 3 | Propionic acid (HPr) | C3H6O2 | Merck KGaA, Darmstadt, Germany | 4.88 | 4.67-7.02 | 54 | 599 (0; 1) | 0-10 | 17 | 558 (0; 2) | Calcium propionate (CaPr) | Riedel-de Haën, Seelze, Germany |
| 2, 3 | Formic acid (HFo) | CH2O2 | Acros Organics, New Jersey | 3.75 | 4.49-7.00 | 39 | 399 (1; 0) | 0-8 | 21 | 599 (0; 1) | Potassium formate (KFo) | Sigma-Aldrich Chemie GmbH, Steinheim, Germany |
a
The first number is the number of data points excluded since at t = 0, the optical density (OD) values were above the detection limit of OD = 0.2. The second number is the number of data points excluded according to the criteria in Materials and Methods.