TABLE 1.
P. chrysogenum strains used in this study
| Strain | Description | Source or reference |
|---|---|---|
| NRRL1951 (ATCC 9480) | Wild-type P. chrysogenum | American Type Culture Collection |
| NRRL1951 GFP-SKL | NRRL1951 expressing GFP-SKL under the control of PgpdA | This study |
| Wis54-1255 (ATCC 28089) | P. chrysogenum with improved PEN production | American Type Culture Collection |
| Wis54-1255 GFP-SKL | Wis54-1255 expressing GFP-SKL under the control of PgpdA | This study |
| S2201 | Non-oleate-utilizing mutant of Wis54-1255 | This study |
| S2202 | Non-oleate-utilizing mutant of Wis54-1255 | This study |
| S2203 | Non-oleate-utilizing mutant of Wis54-1255 | This study |
| S2204 | Non-oleate-utilizing mutant of Wis54-1255 | This study |
| S2201 GFP-SKL | S2201 expressing GFP-SKL under the control of PgpdA | This study |
| S2202 GFP-SKL | S2202 expressing GFP-SKL under the control of PgpdA | This study |
| S2203 GFP-SKL | S2203 expressing GFP-SKL under the control of PgpdA | This study |
| S2204 GFP-SKL | S2204 expressing GFP-SKL under the control of PgpdA | This study |
| DS17690 | High-PEN-producing P. chrysogenum strain | 8 |
| DS17690 GFP-SKL | DS17690 expressing GFP-SKL under the control of PgpdA | This study |
| DS54465 | DS17690 ΔhdfA with increased homologous recombination | 23 |
| DS54465 GFP-SKL | DS54465 ΔniaD::PgpdA-GFP-SKL-TpenDE | This study |
| DS17690 Pex3-GFP DsRed-SKL | DS17690 expressing Pex3-GFP and DsRed-SKL both under the control of PgpdA | This study |
| Δdnm1 GFP-SKL | DS54465 GFP-SKL Δdnm1::amdS | This study |
| DsRed-SKL | DS17690 with DsRed-SKL under the control of PpcbC | 12 |
| Dnm1++++ | DsRed-SKL with integrated multiple copies of a PpcbC-dnm1-His8-TpenDE cassette | This study |