TABLE 1.
Primers used for quantitative PCR
| Primer | Target | Orientation | Sequence |
|---|---|---|---|
| B90111 | Honeybee actin | Forward | AGGAATGGAAGCTTGCGGTA |
| B90121 | Honeybee actin | Reverse | AATTTTCATGGTGGATGGTGC |
| Ntub7025 | N. ceranae tubulin | Forward | AGAACCAGGAACGATGGAGA |
| Ntub7026 | N. ceranae tubulin | Reverse | TCCTTGCAAACAATCTGCAC |
| JCNcer.F | N. ceranae 16S rRNA | Forward | CAATATTTTATTATTTTGAGAGA |
| JCNcer.R | N. ceranae 16S rRNA | Reverse | TATATTTATTGTATTGCGCGTGCA |
| NA70011 | Nc006 (forward) | Forward | CACCTGAAAACAACTTACCTAC |
| NA70021 | Nc006 (reverse) | Reverse | GTATCTTGCCTTACCATCAC |
| NA70031a | Nc123 (forward) | Forward | GGAAAAGATGAGAATATGGAAGAAG |
| NA70041b | Nc123 (reverse) | Reverse | CCAGTTACCCTTGTTTGTGTAGG |