TABLE 1.
Bacterial strains and vectors used in this study
| Strain or vector | Relevant characteristic or genotype | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| EPI300-T1R | F−mcrA Δ(mrr hsdRMS mcrBC) φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara leu)7697 galU galK λ−rpsL nupG trfA tonA dhfr | Epicentre |
| OP50 | Uracil auxotroph | 9 |
| P. tunicata D2 | Marine isolate | 29 |
| P. aeruginosa PA01 | Gene cluster hcnABC, involved in the synthesis of the toxic compound hydrogen cyanide | 18 |
| Vectors | ||
| pCC1FOSa | Fosmid backbone for P. tunicata D2 library; Cmr | Epicentre |
| p519ngfp | High-copy-number plasmid with constitutive GFP expression; Kmr | 54 |
| pBAD-TOPO ZP_01132244a | D2 wild-type gene ZP_01132244 cloned downstream the PBAD promoterb; Ampr | This study |
a
Copy number inducible by arabinose.
b
According to the manufacturer's protocol (pBAD TOPO TA expression kit; Invitrogen).