Fig. 3.

Alterations in the cell number (A) and size (B) of c9, t11-CLA-treated 3T3-L1 adipocytes. The differentiation of 3T3-L1 preadipocytes was initiated 2 days after confluence for 3 days in growth medium containing 0.25 µM dexamethasone, 0.5 mM IBMX, and 1 µg/ml insulin. This was followed by 2 days in growth medium containing 1 µg/ml insulin. Thereafter, the cells were cultured in the growth medium for 2 days. c9, t11-CLA (100 µM) was added to the medium from day-3 (time of addition of dexamethasone, IBMX, and insulin) to day-9 (end point of the experiment). The treated cells were fixed in 4% formaldehyde-phosphate buffer and stained with 0.3% Oil Red O dye. Within 1,300 mm² of the microscopic pictures, the total amount (A) and number of cells less than 29 µm, from 30 to 45 µm, or more than 46 µm (B) of the Oil Red O-stained cells in the presence or absence of c9, t11-CLA were assessed. The data represent the means ± SE. from four experiments. *p<0.01 vs Control. IBMX, 3-isobutyl-1-methyl-xanthine.