Fig. 5.

The effects of the PPARγ agonist rosiglitazone and antagonist GW9662 on the TG contents (A) and Oil Red O staining (B) of 3T3-L1 adipocytes. The differentiation of 3T3-L1 preadipocytes was initiated 2 days after confluence for 3 days in growth medium containing 0.25 µM dexamethasone, 0.5 mM IBMX, and 1 µg/ml insulin. This was followed by 2 days in growth medium containing 1 µg/ml insulin. Thereafter, the cells were cultured in the growth medium for 2 days. Rosiglitazone (1 µM) or GW9662 (5 µM) was added to the medium from day-3 (time of addition of dexamethasone, IBMX, and insulin) to day-9 (end point of the experiment). (A): The treated cells were lysed with lysis buffer, and the TG contents were measured using a Triglyceride E-test Wako kit. The data represent the means ± SE. from four experiments. *p<0.01 vs Control. (B): The treated cells were fixed in 4% formaldehyde-phosphate buffer and stained with 0.3% Oil Red O dye. PPARγ, peroxisome proliferator-activated receptor γ; IBMX, 3-isobutyl-1-methyl-xanthine; TG, triacylglycerol.