Fig. 3.

Folic acid supplementation increases expression of Notch1 and Hes5 mRNAs. Neural stem cell (NSC) neurospheres were incubated for 6 days in medium containing 4 mg/l folic acid (Control), 8 mg/l folic acid (Folate-L), or 44 mg/l folic acid (Folate-H). Subsequently Notch1 and Hes5 mRNA levels were determined by real-time PCR and normalized to β-actin expression. Shown are mean ± SD values of 3 experiments. ^ap<0.05 vs Control, ^bp<0.05 vs Folate-L.