Table 4.
Relative expression of hepatic pro-inflammatory cytokines in rats fed C, HF or HF + NAC diets for 65 d1
Gene | C (n=4) | HF (n=7) | HF + NAC (n=8) |
---|---|---|---|
TNF-α (fold of control) | 1.0 ± 0.40a | 13.3 ± 0.98b | 4.2 ± 1.20a |
IL-1β (fold of control) | 1.0 ± 0.17 | 2.6 ± 0.76 | 2.5 ± 0.26 |
IL-12 (fold of control) | 1.0 ± 0.29 | 1.0 ± 0.24 | 0.7 ± 0.32 |
Values are means ± SEM, relative expression of target gene mRNA normalized to cyclophilin A mRNA as a housekeeping gene using real time RT-PCR relative to mean value for C group = 1.0. Means in a row without a common letter differ, p≤0.05.