Figure 2.

Stimulation of THP-1 cells with CypA induced the expression of IFI27 and IFITM1. (a) THP-1 cells were stimulated with 0.1 μM of CypA for 20 hr. Total cellular RNA was then isolated for real-time RT-PCR with primers specific for IFI27, IFITM1, or GAPDH. Ct values obtained from IFI27 or IFITM1 amplification curves were normalized with that of GAPDH. (b) THP-1 cells were stimulated and cellular RNAs were isolated as in (a). RT-PCR analysis was performed with primers specific for IFI27, IFITM1, or GAPDH and the products were run on agarose gel. These experiments were repeated three times with essentially the same results.