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Non-reducing 12% SDS PAGE of dissolved crystals of the C47A/A241C c6 enzyme. A non-reducing SDS PAGE of a single crystal of the C47A/A241C c6 enzyme washed and dissolved in 100 mM Tris-acetate buffer pH 8.3. Lane 1, wild-type E. coli ATCase holoenzyme; Lane 2, C47A/A241C c6 solution before crystallization; Lane 3, C47A/A241C c6 crystal dissolved in buffer.
