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. Author manuscript; available in PMC: 2010 Nov 13.
Published in final edited form as: Prog Neuropsychopharmacol Biol Psychiatry. 2009 Aug 12;33(8):1347–1355. doi: 10.1016/j.pnpbp.2009.08.003

Fig. 1.

Fig. 1

A) Extracellular recordings from the jcBNST in coronal brain slices of Wistar rats. Field potential recordings were carried out from the jcBNST in acute brain slices as previously described (Francesconi et al., 2009) and as shown in Fig. 1A. st: stria terminalis; jcBNST: juxtacapsular bed nucleus of the stria terminalis (shown in yellow); BNSTdl: dorsolateral bed nucleus of the stria terminalis; BNSTdm: dorsomedial bed nucleus of the stria terminalis; BNSTv: ventral bed nucleus of the stria terminalis; ac: anterior commissure; LV: lateral ventricle; ic: internal capsule. Modified from Paxinos and Watson (Paxinos and Watson, 1998). B) The D1-class receptors antagonist SCH23390 impairs LTP-IE in the jcBNST when applied during the induction paradigm. Field potential amplitude of slices that received a 30 min application (horizontal bar) of SCH23390 (2μM; open squares; n= 7) during delivery of HFS to the stria terminalis impairs LTP-IE induction. Field potential amplitude of control slices that received the same tetanization paradigm are shown (n = 7, filled squares). Application of the D2 receptor blocker sulpiride (50μM; n= 5) did not block the induction of LTP-IE (**SCH23390 different from Control and Sulpiride, p<0.01). C) Catecholamine depletion decreases LTP-IE in the jcBNST. Rats were treated with reserpine (5 mg/kg i.p.) 6 h before sacrifice to deplete endogenous catecholamines or vehicle. Comparison of LTP-IE in control (n= 7, filled square) and depleted (n= 4, open circle) jcBNST slices showed a decrease of the magnitude of LTP-IE after catecholamine depletion (**=p<0.01). D) The D1-class agonist SKF81297 substantially restores LTP-IE in catecholamine depleted slices. The D1 agonist SKF81297 (10 μM), applied 15 min before HFS for 30 min, facilitated LTP-IE induction in depleted slices (n=4, filled triangle). LTP-IE in depleted slices is shown for comparison (n=4, open circle) (*=p<0.05-0.01).