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Lag phase comparison of 15-hLO-1 with AA (5 μM) (open triangles) and LA (5 μM) (open circles). Pre-incubation with AA (25 μM) followed by LA (5 μM) addition (closed circles), and pre-incubation of LA (25 μM) followed by AA (5 μM) addition (closed triangles). Enzymatic assays were performed in 25 mM Hepes buffer (pH 7.5, 22 °C). Insert: Magnified view of lag phase data.
