. Author manuscript; available in PMC: 2010 Sep 7.

Published in final edited form as: Biochemistry. 2009 Jul 7;48(26):6259–6267. doi: 10.1021/bi802332j

Table 2.

Energy of Activation and Arrhenius Prefactors Determination for Human Lipoxygenase Deuterium Abstraction^a

	Substrate	^Dk_cat^b	E_act (kcal/mol)	A_D (s^-1)
12-hLO	AA	12.0 (2.0)	11.9 (1.0)	4 × 10⁸
15-hLO-1	LA^c	40.0 (8.0)	8.7 (0.2)	4 × 10⁵

Data were collected between 15 – 40 °C in 25 mM Hepes pH 7.5 for 15-hLO-1, and 25mM Tris pH 7.5 for 12-hLO.

^Dk_cat = k_cat^H/k_cat^D at 30 °C.

From previously published data (18).