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. 2001 Feb 13;98(4):1895–1900. doi: 10.1073/pnas.98.4.1895

Figure 1.

Figure 1

Accumulation of viral DNA in cells infected with UL97 mutant RCΔ97.08 (Δ97) and parental strain AD169. (A) DNA accumulation curves of DNA copies per well from QC-PCR (0, 24, 48 hpi) and blot hybridization (72, 96, 168 hpi) after infection of HFs with an moi of 1. ○, AD169; ■, RCΔ97.08. (B) DNA blot hybridization to detect levels of viral DNA after a 96-h PFA-induced DNA synthesis block (D) and release (R) for a subsequent 24, 48, 72, or 96 h. Total cellular DNA was digested with BamHI and blot hybridized to detect a 2.3-kb viral BamHI fragment.