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DNA blot hybridization showing accumulation and cleavage of viral DNA in cells infected with UL97 mutant RCΔ97.08 (Δ97) and parental strain AD169 (moi of 0.1) after BDCRB drug block (D) and release (R) at the indicated times. Total cellular DNA was digested with EcoRI and subjected to blot analysis to detect L-S junction (upper arrow) and L terminal (lower arrow) fragments, with the use of radiolabeled probe pON227.
