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. 2001 Feb 13;98(4):1895–1900. doi: 10.1073/pnas.98.4.1895

Figure 5.

Figure 5

Analysis of total and encapsidated viral DNA in AD169-, RCΔ97.08-, and RCΔ97.19-infected cells. (A) DNA blot hybridization showing accumulation of total and DNase-resistant viral DNA in cells infected with mutant RCΔ97.08 (Δ97) and parental strain (moi of 1). Total infected cell DNA was isolated before or after DNase digestion (+DNase) at the indicated times (hpi). Viral DNA digestion and hybridization were carried out to identify junction and terminal EcoRI fragments (arrows) as indicated for Fig. 3. M, mock-infected. (B) Quantitative analysis of total and DNase-resistant (+DNase) viral DNA in AD169-, RCΔ97.08-, and RCΔ97.19- (Δ97) infected cells at the times indicated. Quantitation was carried out with the use of blot hybridization for all samples, except for the DNase-resistant mutant DNA, and QC-PCR was used for all samples, yielding values similar to blots at higher DNA levels. The mean DNA copy number values, derived from three independent experiments, are displayed. Empty bars, total AD169 DNA; stippled bars, DNase-resistant AD169 DNA; black bars, total RCΔ97 strains DNA; hatched bars, DNase-resistant RCΔ97 strains DNA.