Fig. 3.

Gene expression comparison between normal and Bcl11b-deleted cells in early T cell development in vitro. Subsets of control and Bcl11b-deleted cells were sorted both after primary 9-day culture and after secondary culture as indicated. For sorting gates and quantitation of expression levels in fixed units, see fig. S7. “DN4” cells generated by controls in secondary culture may include later stages. Real-time quantitative polymerase chain reaction data are shown as a heat map normalized to levels of each gene in control DN2a cells. Color scale units are log₁₀ for a 10⁴ overall range. Vertical bars on right highlight gene groups of interest. Results are from one of four representative experiments (independent experiments are shown in figs. S8 and S9).