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. Author manuscript; available in PMC: 2011 Oct 15.
Published in final edited form as: Biochem Pharmacol. 2010 Jun 30;80(8):1238–1251. doi: 10.1016/j.bcp.2010.06.040

Figure 3. Time Courses for the Inhibition of ACh-Stimulated 86Rb+ Efflux Elicited by Exposure to Epibatidine, Cytisine, Nicotine or Methylcarbachol.

Figure 3

Mouse thalamic synaptosomes loaded with 86Rb+ were exposed to two concentrations of the each agonist for the indicated times (0 to 8 min) and then stimulated by exposure to either 30 μM ACh (filled symbols: ●lower agonist concentration; ■ higher agonist concentration) or 1000 μM ACh (open symbols: ○lower agonist concentration; □ higher agonist concentration). The agonist concentrations used for exposure prior to stimulation were: nicotine; 10 nM and 200 nM; cytisine; 20 nM and 200 nM; epibatidine 0.3 nM and 3 nM and methylcarbachol; 100 nM and 500 nM. Each data point represents the mean±SEM of 3-5 separate experiments. Curves were determined by nonlinear least squares fitting of the data as an exponential decay with residual activity as described in the methods.