. Author manuscript; available in PMC: 2011 Oct 15.

Published in final edited form as: Biochem Pharmacol. 2010 Jun 30;80(8):1238–1251. doi: 10.1016/j.bcp.2010.06.040

Table 3.

Maximal HS and LS ACh-Stimulated ⁸⁶Rb⁺ Efflux and Cytisine-Sensitive [¹²⁵I]Epibatidine Binding in Cortex and Thalamus of Mice Chronically Treated with Nicotine

Nicotine Infusion Dose	0.0 mg/kg/hr	0.5 mg/kg/hr	4.0 mg/kg/hr
CORTEX
High Sensitivity ⁸⁶Rb⁺ Efflux	2.11±0.11	2.06±0.19	1.88±0.11
Low Sensitivity ⁸⁶Rb⁺ Efflux	6.81±0.59	6.80±0.50	5.63±0.80
[¹²⁵I]-Epibatidine Bound	88.2±0.7	127.9±1.1	139.0±1.2
THALAMUS
High Sensitivity ⁸⁶Rb⁺ Efflux	13.07±0.89	10.19±0.99	6.76±1.19
Low Sensitivity ⁸⁶Rb⁺ Efflux	16.26±1.27	13.84±2.48	12.52±1.96
[¹²⁵I]-Epibatidine Bound	198.4±2.7	240.7±2.5	256.8±2.9

HS and LS ACh-stimulated ⁸⁶Rb⁺ efflux were calculated as described in the Methods. Values represent mean ± SEM for 9-11 individual experiments for Cortex and 7 individual experiments for Thalamus. ⁸⁶Rb⁺ efflux data are presented as total efflux normalized to baseline. [¹²⁵I]Epibatidine binding is given as fmol/mg protein.