FIG. 4.

Decreased protein abundance of DNA glycosylases in retinal mitochondria is not reversed by good glycemic control. Retinal mitochondrial protein (20 μg) was separated on 10% polyacrylamide gels, transferred to nitrocellulose, and analyzed by Western blot using polyclonal antibodies for OGG1 (a) and MYH (b) with Cox IV as a loading control. To evaluate nuclear contamination, the membranes were screened for Histone H2B expression. Histograms represent band intensities of OGG1 and MYH, respectively, normalized to that of Cox IV. The values are presented as the mean + SD of 4 rats in each group. *p < 0.05 compared to normal.