FIG. 9.

Nox-4 delivers H₂O₂ directly into the nucleus of EOMA cells. Catalase (0.1 mU/cell) tagged with Qdot was microinjected to the cytoplasm [(a) catalase; (b) DCF; (c) merged image 30 s after injection; (d) catalase; (e) DCF; (f) merged image 3 min after injection) or the nucleus of an EOMA cell (g) catalase; (h) DCF; (i) merged image 30 s after injection; (j) catalase; (k) DCF; (l) merged image 3 min after injection]. Three minutes after microinjection, catalase-injected cytoplasm (e) and nucleus (k) showed lower DCF intensity (m). Results are expressed as mean ± SD. *p < 0.05; white scale bar, lower right hand corner = 30 μm. Catalase-sensitive DCF signal in the nucleus confirms that the DCF oxidation signal noted was generated by H₂O₂. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article at www.liebertonline.com/ars).