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. 2010 Sep 15;13(6):821–831. doi: 10.1089/ars.2009.3036

FIG. 2.

FIG. 2.

Dose response of TNF-α induced-mitochondrial changes. Transmission electron microscopic investigations of Jurkat cells in monolayer culture (A). The cells were treated with indicated concentrations of TNF-α for 1 h. Degenerative changes in mitochondria (swelling and dilatation) (arrows) increased in Jurkat cells with increasing concentration of TNF-α in the growth medium. Original magnification, × 50,000. (B) Time course of TNF-α-induced mitochondrial changes. Transmission electron microscopic investigations of Jurkat cells in monolayer culture. Jurkat cells were incubated for indicated time intervals with 1 nM TNF-α. Degenerative changes in mitochondria (swelling and dilatation) (arrows) increased in Jurkat cells with increasing time. Original magnification, X 50,000. (C) Effect of TNF-α on mitochondria in Jurkat cells. Jurkat cells were treated with TNF-α (1 nM) for indicated times. The number of cells with mitochondrial changes was determined by scoring 250 cells from 30 different microscopic fields in the electron microscope. The diagram shows the number of pathologically altered cells. Columns, mean from three different experiments; bars, SD. *p < 0.05; **p < 0.01, Student's t test.