Skip to main content
. 2010 Sep 15;13(6):821–831. doi: 10.1089/ars.2009.3036

FIG. 5.

FIG. 5.

Dose-dependent activation of caspase-3 in TNF-α-treated Jurkat cells. (A) Cells were treated with indicated concentrations of TNF for 1 h and labeled with anti-caspase-3 antibodies by alkaline phosphatase-anti-alkaline-phosphatase (APAAP) technique. Treated cells showed activated caspase-3 in their nuclei in response to TNF-α treatment. Untreated cells revealed no caspase-3 activation (original magnification, X 160). (B) Cells were treated with indicated concentrations of TNF-α for 1 h and cell lysates were investigated for activated caspase-3 using anti-caspase-3 antibodies by Western blot analysis.