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. 2010 Sep 7;5(9):e12170. doi: 10.1371/journal.pone.0012170

Figure 8. Effect of olprinone on immunohistochemical localization and expression of Fas-ligand.

Figure 8

At 24 hr after SCI a substantial increase in Fas-ligand expression (B) was found in inflammatory cells, in nuclei of Schwann cells in wm and gm of the spinal cord tissues. Spinal cord Fas-ligand was significantly attenuated in olprinone-SCI treated mice in comparison to SCI animals (C). Moreover, SCI increased the number of S100β immunoreactive astrocytes in the white and grey matters of the spinal cord at caudal levels adjacent to the injury (E). This number of S100β immunoreactive astrocytes is significantly reduced in the spinal cord obtained from oprinone-treated SCI mice (F). Densitometry analysis of immunocytochemistry photographs (n = 5 photos from each sample collected from all mice in each experimental group) for Fas-ligand and S100β from spinal cord tissues was assessed. Similarly, at 24 h after SCI, the expression of Fas-ligand in the spinal cord homogenates was investigated by Western blot. A significant increased Fas-ligand (H) expression was observed in the spinal cord from mice subjected to SCI. On the contrary, olprinone treatment prevented the SCI-induced Fas-ligand (H) expression. This figure is representative of at least 3 experiments performed on different experimental days. wm: White matter; gm: gray matter.