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. 2010 Sep 7;5(9):e12592. doi: 10.1371/journal.pone.0012592

Figure 3. IGF-I treatment impairs autophagy.

Figure 3

WI-38 fibroblasts stably expressing the GFP-LC3 fusion protein were maintained for 14 days in MCDB 105 medium, or MCDB 105 medium with IGF-I (40 ng/ml). Medium with or without growth factor was replenished every 3 days. A. Number of LC3 puncta per cell in WI-38 GFP-LC3 cells with or without 40 ng/ml IGF-I (**, P<0.01) At least 25 fields and 100 cells per slide were examined. B. Representative fluorescence micrograph (40X) of WI-38 GFP-LC3 cells with or without IGF-I treatment. C. Accumulation of LC3 and p62/SQSTM1 over time in IGF-I-treated cells as assessed by western blot. D. Protein degradation in control and IGF-I-treated cells measured as percentage of the residual 35S-Methionine radioactivity at the indicated time points over time 0 (*, P<0.01).