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. 2010 Sep 7;5(9):e12592. doi: 10.1371/journal.pone.0012592

Figure 5. Rapamycin restores autophagy in IGF-I-treated cells.

Figure 5

WI-38 fibroblasts stably expressing the GFP-LC3 fusion protein were maintained for 14 days in MCDB 105 medium, or MCDB 105 medium with IGF-I (40 ng/ml). Medium with or without growth factor was replenished every 3 days. A. Representative fluorescence micrographs of WI-38 GFP-LC3 cells with or without 40 ng/ml IGF-I and/or 10 nM rapamycin. B. Number of LC3 puncta per cell in WI-38 GFP-LC3 cells (*, P<0.05). At least 25 fields and 100 cells per slide were examined C. Levels of LC3 and p62/SQSTM1 in WI-38 with or without 40 ng/ml IGF-I and/or 10 nM rapamycin as assessed by Western blot analysis.