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. 2010 Sep 7;5(9):e12595. doi: 10.1371/journal.pone.0012595

Figure 3. Dual reporters representing different YMC phases show separation of peak expression.

Figure 3

(A) Samples from diploid cells expressing two reporters, an mCherry reporter of an OX phase gene (pDBP2-mCherry) and a GFP reporter of an RC phase gene (pATO3-GFP) were collected from across the YMC at the time points indicated from 1-12. (B) mRNA levels of DBP2 and ATO3 across two consecutive cycles of the YMC as determined previously [5], and western blots showing reporter GFP or mCherry protein levels across the YMC. The time points with highest reporter expression are highlighted by a yellow line. (C) Panels show images of cycling cells expressing pDBP2-mCherry and pATO3-GFP at the indicated time points. (D) A larger field of cells from time point 9, showing the expression levels of the two reporters within the same cell. A partial selection of cells showing opposite expression of red or green reporters within the same cell are highlighted with white arrows. Loading controls for panel B are shown in Figure S1.