Figure 3. The structure and nucleotide sequence of the 5′ end of Lim3A and Lim3C.

Letters in blue correspond to 5′ UTR of Lim3A, letters in red – to translated region of the first exon of Lim3A, Lim3C. Letters in violet correspond to primers for PCR and sequence analysis. Letters in green correspond to primers for Real-time RT-PCR. The borders of the analysed sequence are depicted by </>. The promoter sequences predicted by the Neural Network Promoter Prediction database, version 2.2 are depicted by light grey color. Sequences marked with green rectangles are initiation regions. TSSs are indicated by large bold letters and asterisks. The underlined sequences are putative core promoter elements. Letters outlined by squares are polymorphic sites which were present in the sample with the frequency 0.06 and higher, insertions are depicted by black rectangles. Large, bold letters in italics outlined by squares are lifespan associated SNPs. Large, bold letters in italics outlined by pink squares are SNPs composing haplotype significantly associated with lifespan. Letters outlined by blue squares are SNPs significantly associated with Lim3A mRNA amount. The transcription factor binding sites are denoted by the dotted lines.