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. 2010 Sep 7;4(9):e815. doi: 10.1371/journal.pntd.0000815

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Microtiter wells were coated with 1 µg of human skin elastin and binding was measured by ELISA. (A) PBS-5 mM MgCl₂ (no cells) or 1×10⁸ L. interrogans L1-130 in PBS-5 mM MgCl₂ (cells) without antiserum (no Ab) or in the presence of OmpL37 or OmpL54 antiserum (Ab) diluted 1∶40, 1∶80, 1∶160, 1∶320, and 1∶1280 were added to the elastin-coated wells. (B) Recombinant OmpL37 (0.5 µg) was preincubated with or without the IgG purified from 10 times diluted OmpL37 or OmpL47 antiserum at 1∶40, 1∶160, and 1∶640 dilution and the OmpL37 or OmpL47 antiserum at 1∶2500 dilution prior to addition to elastin-coated wells. Mean absorbance at 450 nm ± the standard deviation of a representative experiment performed in triplicate is shown. Statistical significance was evaluated by one-way ANOVA comparing leptospiral or recombinant OmpL37 binding in the presence of antibodies compared with binding in the absence of antibodies (** P<0.001, * P<0.05).