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. 2010 Sep 7;4(9):e815. doi: 10.1371/journal.pntd.0000815

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Recombinant OmpL47, OmpL37, and OmpL36 were separated by gel electrophoresis (2.5 µg per lane), stained with Coomassie G-250 (A) or blotted onto PVDF membrane, and analyzed for serum reactivity. (B) Membranes probed with a pool of two L. interrogans L1-130-infected hamster sera (1∶1000) or non-infected control hamster serum (1∶1000). (C) Membranes probed with normal, acute, or convalescent pooled human sera (1∶300). The positions of molecular mass standards (in kilodaltons) are indicated on the left.