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. 2010 Sep 7;4(9):e815. doi: 10.1371/journal.pntd.0000815

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Extracts of 5×10⁷ leptospires per lane were separated by gel electrophoresis, blotted onto PVDF membrane, and probed with OmpL37 antiserum (1∶1000) (A) or stained with Coomassie G-250 (B). L. interrogans serovar Copenhageni strain Fiocruz L1-130 (Lane 1), L. interrogans serovar Pomona strain PO-01 (Lane 2), L. kirscheneri serovar Mazdok strain 5621 (Lane 3), L. kirscheneri serovar Grippotyphosa strain RM 52 (Lane 4), L. weilii serovar Celledoni strain Celledoni (Lane 5), L. biflexa serovar Patoc strain Patoc 1 (Lane 6), L. wolbachii serovar Biflexa strain codice (Lane 7), Leptonema illini strain 3055 (Lane 8), Leptospira inadai serovar Lyme strain 10 (Lane 9), L. borgpetersenii serovar Tarrasovi strain Perepelicin (Lane 10), L. borgpetersenii serovar Javanica strain Veldrat Bataviae 46 (Lane 11). The saprophytic species in lanes 5 to 9 are indicated by asterisks. The positions of molecular mass standards (in kilodaltons) are indicated on the left.