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. 2010 Sep 7;8(9):e1000476. doi: 10.1371/journal.pbio.1000476

Figure 2. ERK regulates TPO-induced senescence.

Figure 2

UT711oc1 cells were cultured for 5 d in presence of TPO and 10 µM of PD98059 or U0126 MAPK inhibitor. MAPK inhibitors (a) restore cell proliferation, (b) increase BrdU incorporation, (c) decrease SA-β-galactosidase activity, and (d) inhibit ERK phosphorylation and p21 protein expression. UT711oc1 cells were transduced with either empty retroviral vector (pMigr) or the vector encoding for a spontaneously active MEK (MEK1-SS/DD). MEK1-SS/DD (e) inhibits GM-CSF-dependent cell proliferation, (f) blocks BrdU incorporation, (g) induces SA-β-galactosidase staining, and (h) causes an increase in ERK phosphorylation and p21 protein expression. Error bar represents the standard deviation of three independent experiments.