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Single turnover excision of Hx by AAG. Representative data for select substrates are shown. (A) The results for the symmetric 35 (□) and 49 (⋄) sequences are compared to previously published results for the 25 (•) sequence (8). (B) The results for asymmetric substrates that were shortened at either the upstream (19u, ○) or downstream (19d, □) flanking region. The average of 2–3 independent reactions is shown and the error bars indicate the standard deviations.
