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. Author manuscript; available in PMC: 2010 Sep 7.
Published in final edited form as: Biochemistry. 2010 Sep 14;49(36):7867–7878. doi: 10.1021/bi100797z

FIGURE 3.

FIGURE 3

Analysis of base analogues in TFOs containing 2′-OMe ribose. (a) Bases and analogues in TFOs: 1, 2′-OMe thymidine; 2, 2′-OMe 5Me cytosine. (b) Binding assay of analogues shown in panel a. Resin-bound TFOs with base analogues were incubated with hairpin duplexes containing each of four possible base pairs at the X:Y position shown in Figure 2. After being washed, the remaining bound duplex was eluted from the resin (Methods) and the amount measured spectrophotometrically. (c) Thermal stability of 2′-OMe TFOs containing T (against the wild-type target sequence) or analogue 3c in the Z position.