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. Author manuscript; available in PMC: 2010 Sep 7.
Published in final edited form as: Biochemistry. 2010 Sep 14;49(36):7867–7878. doi: 10.1021/bi100797z

FIGURE 5.

FIGURE 5

Bioassay of TFO with the 5a analogue. (a) Construction of the CHO cell line with the murine triplex target site adjacent to exon 5 of Hprt. A cell line with a variant triplex target sequence adjacent to exon 5 was constructed by targeted sequence conversion (55, 56). This cell line is isogenic to the CHO AA8 line, differing only in the conversion of an A:T duplex to a C:G duplex at the X:Y position as shown in Figure 2. (b) Frequency of Hprt deficient colonies after treatment with AE-TFO-T and AE-TFO-5a in cells with the wild-type (A:T) and triplex (C:G) target.