Skip to main content
. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Cell Immunol. 2010 Jul 17;265(1):65–73. doi: 10.1016/j.cellimm.2010.07.004

Figure 5. Real time RT-PCR validation of leading edge genes from the “Stemcell_Common_Up” gene set.

Figure 5

cDNA was generated from the CD8+ T cell of tumor-bearing and tumor-free mice treated with anti-CD25 and vaccine. cDNA was examined for the expression of probe sets representing leading edge genes of the “Stemcell_Common_Up” gene set by real time RT-PCR. Results were calculated using the ΔΔCt method with 18S rRNA as the internal reference and cDNA from CD8+ T cells from naïve (tumor-free, non-treated) mice as the calibrating sample. Results were generated using the same RNA samples used in one of the microarray experiments and are representative of two experiments using RNA samples from groups of mice from independent experiments.