. Author manuscript; available in PMC: 2011 Sep 14.

Published in final edited form as: Biochemistry. 2010 Sep 14;49(36):7902–7912. doi: 10.1021/bi1009375

Table 5.

Class III Substrates of H_peroxo and Q

Substrate	Species	k_sat (s^-1)	K_M (mM)	k_init (M^-1 s^-1)^a	KIE_app^b
CH₃CH₂CHO	H_peroxo	6.0 ± 0.2	44 ± 4	85.5 ± 0.1	nd^c
CH₃CHO	H_peroxo	7.6 ± 0.2	43 ± 3	110.08 ± 0.03	nd^c
HCOONa	H_peroxo	0.71 ± 0.01	458 ± 65	0.91 ± 0.01	nd^c
CH₃CN	H_peroxo	0.2727 ± 0.0003	39 ± 6	2.93 ± 0.01
CD₃CN	H_peroxo	0.352 ± 0.001	78 ± 3	2.02	0.775 ± 0.002
CH₃NO₂	H_peroxo	0.912 ± 0.002	403 ± 11	nd^c	na^d

CH₃CN	Q	126 ± 8	617 ± 71	180 ±1
CD₃CN	Q	2.02 ± 0.03	130 ± 3	12.4	62 ± 4
CH₃NO₂	Q	1.54 ± 0.2	13 ± 1	nd^c
CD₃NO₂	Q	0.005 ± 0.0005	11 ± 5	nd^c	31 ± 3

k_init values were calculated by fitting the linear portion of the curve at low substrate concentration to eq 1.

Apparent kinetic isotope effect, k_sat,H/k_sat,D.

Not determined.

Not applicable. For CD₃NO₂, KIE_app could not be determined for the reaction of H_peroxo because k_obs displayed a linear dependence on substrate concentration.