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. Author manuscript; available in PMC: 2011 Sep 14.
Published in final edited form as: Biochemistry. 2010 Sep 14;49(36):7902–7912. doi: 10.1021/bi1009375

Table 7.

Apparent KIEs and Correlation of ksat with Thermodynamic Parameters for Class III Substrates of Q at 4 °C and °20 C

Substrate T (°C) ksat (s-1) KIEappa D(RH) (kcal/mol)b IP (eV) pKac
CH3CN 4 126 ± 8 97.0 12.20d 25.0
20e 282 ± 10
CD3CN 4 2.02 ± 0.03 62 ± 4
20e 6.1 ± 0.1 46.4 ± 2.3
CH3NO2 4 1.54 ± 0.02 96.3 11.28f 10.2
20e 5.34 ± 0.02
CD3NO2 4 0.050 ± 0.0005 31 ± 3
20e 0.66 ± 0.2 8.1 ± 0.2
a

Apparent kinetic isotope effect, ksat,H/ksat,D.

b

Data from (47).

c

Data from (49).

d

Data from (67).

e

Data from (23).

f

Data from (68).