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. 2010 Sep 6;190(5):927–940. doi: 10.1083/jcb.201006105

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© 2010 Craige et al.

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Figure 4. — Immunogold localization of CEP290. (A) Wild-type detergent-extracted cytoskeletons were incubated with CEP290 antibody and gold-conjugated secondary antibody, then fixed, embedded, and sectioned. The locations of 34 gold particles from ∼30 sections are indicated by black dots superimposed on the EM. For simplicity, the black dots are depicted on only one side of the transition zone; no bias for either side of the transition zone was observed. A selection of original micrographs is shown in Fig. S3. (B and C) cep290::CEP290-HA cytoskeletons were double-labeled with antibodies to CEP290 (12-nm gold) and HA (6-nm gold). As in A, the locations of the particles from several longitudinal sections (B) and cross sections (C) are represented by black dots superimposed on a single EM. A selection of original micrographs is shown in Fig. S4. Bars, 100 nm. (D) Schematic of HA-tagged CEP290 depicting the locations of the HA and C-terminal epitopes.